Bordetella pertussis ga qarshi antikorlar, IgM
Kod:17033
Tahlil ma'lumotlari
Tadqiqot usuli
- Ferment bilan bog'langan immunosorbent analiz (ELISA)
Kutilayotgan natija topshirish vaqti
1–2 kun
Maxsus tayyorlik
- Do not smoke for at least 30 minutes before the blood draw.
Qanday foydalanish
Bordetella pertussis ga qarshi antikorlar, IgM (shuningdek Anti-Bordetella pertussis IgM yoki Pertussis IgM antibodies nomlari bilan ham yuritiladi) patogenga xos IgM ni aniqlash orqali koklyushning serologik tashxisiga yordam beradi. U simptomlar boshlanganidan taxminan ikkinchi haftadan boshlab o'tkir yoki davom etayotgan infeksiyani tanib olishni qo'llab-quvvatlaydi va ayniqsa emlanmagan bolalarda alohida foydali. Ushbu test klinik va epidemiologik ma'lumotlar bilan birgalikda talqin qilinadi va infeksiyani tasdiqlash uchun ko'pincha ekish va PCR kabi bevosita aniqlash usullari bilan juft qo'llaniladi.
Cheklovlar
Koklyush aerob, gram-manfiy kokkobatsilla Bordetella pertussis tomonidan chaqiriladigan insonga xos nafas yo'llari kasalligi bo'lib, nafas tomchilari orqali tarqaladi. Kasallik inkubatsiya, kataral, paroksizmal va rekonvalestsent bosqichlardan o'tadi; paroksizmal fazada spazmodik yo'tal, inspirator hushtaksimon ovoz va yo'taldan keyingi qusish xosdir. Absolyut limfositoz bilan birga leykotsitoz tez-tez kuzatiladi. Xo'jayin antikor javobini chaqiradigan asosiy antigenlarga pertussis toksini, filamentoz gemaglyutinin, pertaktin, tashqi membrana oqsillari, lipopolisaxarid va agglutinogenlar kiradi. Gumoral javoblar vaqt o'tishi bilan rivojlanadi: IgA va IgM odatda simptomlar boshlanishidan keyingi ikkinchi haftada paydo bo'ladi, IgG esa 6–8-haftalarda cho'qqiga yetadi va so'ng susayadi. Bordetella pertussis ga xos IgM ning yuqoriligi, yakka o'zi yoki IgA va/yoki IgG bilan birga aniqlanishi, ayniqsa avval emlanmagan shaxslarda o'tkir yoki yaqinda kechgan infeksiyani qo'llab-quvvatlaydi. Juda erta namuna olish yoki immun funksiyaning o'zgarganligi haqiqiy infeksiyaga qaramay IgM past bo'lishiga olib kelishi mumkin. Serologiya ekish va PCR ga qo'shimcha usul sifatida xizmat qiladi va natijalar klinik ko'rinish hamda epidemiologik ta'sirlanish kontekstida talqin qilinishi lozim.
| Referens oraliq |
| ||||
|---|---|---|---|---|---|
| Ko'rsatmalar | Clinical picture suggestive of pertussis, including paroxysmal cough with inspiratory whoop, posttussive vomiting, or cyanosis, often with normal or low-grade fever and lymphocytic leukocytosis, Workup of suspected Bordetella pertussis infection when testing occurs at or after 2 weeks from symptom onset, Evaluation of individuals with close exposure to a laboratory-confirmed pertussis case, Differential diagnosis of paroxysmal cough syndromes versus viral upper respiratory infection, measles, acute bronchitis, pneumonia, or asthma |
Namunangiz talablari
| Namunangiz | Zardob |
|---|---|
| Container | Oltin/yo'lbars qopqoqli probirka (SST, gel ajratgich) |
| Saqlash tayyorlik | Sovutilgan, Muzlatilgan |
References
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Piekarska K, Rzeczkowska M, Rastawicki W, Dąbrowska-Iwanicka A, Paradowska-Stankiewicz I. Usefulness of laboratory methods in diagnosis of pertussis in adult with paroxysmal cough. Przegl Epidemiol. 2014;68(4):633-6.
Guiso N, Berbers G, Fry NK, He Q, Riffelmann M, Wirsing von König CH; EU Pertstrain group. What to do and what not to do in serological diagnosis of pertussis: recommendations from EU reference laboratories. Eur J Clin Microbiol Infect Dis. 2011 Mar;30(3):307-12.
Laboratory manual for the diagnosis of whooping cough caused by Bordetella pertussis and Bordetella parapertussis. World Health Organization. Dept. of Immunization, Vaccines and Biologicals. WHO/IVB/04.14, 2004.
Respiratory infections. Diagnosis of pertussis and parapertussis. Methodological guidelines MR 3.1.2.0072-13.