Mycoplasma pneumoniae, DNK PZR
Kod:18009|CPT:87581|LOINC:29257-3
| Kabi | Mycoplasma pneumoniae, PZR |
|---|
Tahlil ma'lumotlari
Tadqiqot usuli
- Real vaqt polimeraza zanjir reaktsiyasi (RT-PCR)
- Polimeraza zanjir reaktsiyasi (PCR)
Kutilayotgan natija topshirish vaqti
1–2 kun
Maxsus tayyorlik
- For sputum collection, drink a large amount of water 8–12 hours before providing the specimen.
- Collect an early-morning, freely expectorated sputum sample after deep coughing; avoid saliva and nasopharyngeal secretions.
- For an oropharyngeal swab, avoid eating or drinking for 3–4 hours beforehand and do not brush teeth, gargle, chew gum, or smoke during that interval.
- For a nasal or nasopharyngeal swab, do not use nasal drops or sprays and do not rinse the nose for 3–4 hours before collection.
- When possible, collect the specimen in the morning.
- Do not smoke during the 30 minutes immediately before specimen collection.
Qanday foydalanish
Mycoplasma pneumoniae, DNK PZR testi (M. pneumoniae DNK si uchun real vaqt PZR) M. pneumoniae ni, jumladan noan'anaviy pnevmoniyani o'z ichiga olgan quyi nafas yo'llari kasalligi bo'yicha tekshiruvdan o'tayotgan bemorlarda aniqlash uchun qo'llanadi. Patogen DNK ni aniqlash antibakterial terapiyaga javobni baholashni qo'llab-quvvatlash uchun ham foydalanilishi mumkin. O'z vaqtida etiologik tashxis talab qilinganda, PZR mikoplazma tekshiruvi uchun madaniylashtirish yoki serologiyaga nisbatan tez va aniq muqobil usulni taklif etadi.
Cheklovlar
Mycoplasma pneumoniae respirator mikoplazmozning tez-tez uchraydigan sababchisi va noan'anaviy pnevmoniyaning yetakchi etiologiyasidir, holatlarning hatto 30% gacha bo'lgan qismini tashkil etadi. Yuqtirish simptomli shaxslar yoki asemptomatik tashuvchilardan respirator tomchilar orqali sodir bo'ladi, inkubatsiya davri taxminan 2–3 hafta. Klinik ko'rinish asemptomatik infeksiyadan tortib isitma, holsizlik, bosh og'rig'i va asta-sekin kuchayib boruvchi yo'talgacha bo'lgan diapazonda bo'lishi mumkin. Taxminan 5%–10% bemorda traxeobronxit yoki pnevmoniya rivojlanadi; u doimiy balg'am bilan xarakterlanadi, balg'amni chiqarish qiyin bo'lishi mumkin va ba'zan qon izlari bilan bo'ladi. Ekstrapulmonar namoyonlar kuzatilishi mumkin va quyidagilarni o'z ichiga oladi: Stivens–Djonson sindromi va boshqa dermatologik topilmalar; aseptik meningit, meningoensefalit va ko'ndalang miyelit; Giyen–Barre sindromi va polinevropatiya; Reyno fenomeni; aplastik anemiya; hamda aritmiyalar, o'tkazuvchanlik buzilishlari va yurak yetishmovchiligi kabi kardial asoratlar. Real vaqt PZR patogenga xos DNK ketma-ketliklarini nishonga oladi va M. pneumoniae ni madaniylashtirish yoki serologik yondashuvlar bilan solishtirganda aniqlash uchun sezgir, tezkor usulni ta'minlaydi.
| O'lchov birligi | qualitative |
|---|---|
| Referens oraliq | — |
| Ko'rsatmalar | Workup of suspected Mycoplasma pneumoniae involvement in lower respiratory tract disease, Evaluation of community-acquired atypical pneumonia when M. pneumoniae is a diagnostic consideration |
Namunangiz talablari
| Namunangiz | Balg'am |
|---|---|
| Container | Steril balg'am idishi |
| Hajm | 0.5 mL (min 0.2 mL) |
| Saqlash tayyorlik | Xona harorati, Sovutilgan, Muzlatilgan |
References
Infectious Diseases: National Guideline. Eds. N.D. Yushchuk, Yu.Ya. Vengerov. Moscow: GEOTAR-Media; 2009. 1056 p.
Baum SG. Mycoplasma pneumoniae and atypical pneumonia. In: Mandell GL, Bennett JE, Dolin R, eds. Principles and Practice of Infectious Diseases. 6th ed. Philadelphia: Churchill Livingstone; 2005.