Epstein–Barr virusi (EBV) DNKsi, real vaqtli PZR
Kod:18042
| Kabi | EBV DNKsi |
|---|
Tahlil ma'lumotlari
Tadqiqot usuli
- Real vaqt polimeraza zanjir reaktsiyasi (RT-PCR)
Kutilayotgan natija topshirish vaqti
1–2 kun
Maxsus tayyorlik
- For 3–4 hours before an oropharyngeal (throat) swab, avoid all food and drink, do not brush teeth, do not rinse the mouth or throat, do not chew gum, and do not smoke.
- For 3–4 hours before a nasal swab, do not use nasal drops or sprays and do not rinse the nose.
- Collect swab specimens in the morning, immediately after overnight sleep, when feasible.
Qanday foydalanish
Epstein–Barr virusi (EBV) DNKsi, real vaqtli PZR sinovi (EBV DNK PZR yoki EBV PZR deb ham ataladi) serologik markerlar hali paydo bo‘lmaganida EBV DNK borligini ko‘rsatish uchun qo‘llanadi va infeksion mononukleozning erta tashxisini qo‘llab-quvvatlaydi. Shuningdek, gerpesvirus bilan bog‘liq yuqori nafas yo‘llari kasalliklarining, jumladan tonsillofaringitning, differensial bahosiga hissa qo‘shadi. Immuniteti susaygan populyatsiyalarda—masalan, transplant qabul qilganlar va kuchli immunosupressiya olayotgan shaxslarda—ushbu tahlil EBV reaktivatsiyasini baholashni qo‘llab-quvvatlaydi. Bunday holatlarda EBV DNKsi aniqlanishi klinik korrelyatsiyada EBV bilan bog‘liq kasallik xavfi va faolligi haqida ma’lumot beradi.
Cheklovlar
Epstein–Barr virusi, Herpesviridae oilasiga mansub bo‘lib, asosan B-limfotsitlarni nishonga oladi, biroq T-limfotsitlar va epitelial hujayralarni ham jalb qilishi mumkin. Yuqish ko‘pincha nafas yo‘llari sekretlari orqali sodir bo‘ladi. Birlamchi infeksiya odatda bolalikda orttiriladi va ko‘pincha simptomsiz yoki minimal simptomlar bilan kechadi. O‘smirlar va kattalarda esa ko‘proq infeksion mononukleoz ko‘rinishida namoyon bo‘ladi; u isitma, holsizlik, limfadenopatiya va tonsillofaringit bilan tavsiflanadi, gepatosplenomegaliya mumkin. EBV infeksiyasi asoratlari qatoriga gepatit, pnevmoniya, gemolitik anemiya, trombotsitopeniya, aplastik anemiya, taloqning yorilishi, miokardit va nevrologik sindromlar (jumladan Giyen–Barre sindromi, ensefalit va meningit) kiradi. Virus xotira B-limfotsitlar va epitelial hujayralarda umrboqiy latentlikni o‘rnatadi. Immunosupressiya davrida (masalan, HIV infeksiyasi yoki transplantatsiyadan keyingi terapiya chog‘ida) reaktivatsiya limfoproliferativ kasalliklar va nazofaringeal karsinoma bilan bog‘liq bo‘lib, mononukleozga o‘xshash qaytalanuvchi kasallik ko‘rinishida namoyon bo‘lishi mumkin. Polimeraza zanjir reaksiyasi (PZR) nishon ketma-ketlikni amplifikatsiya qilish orqali EBV DNKni aniqlaydi. Namuna olish yetarli bo‘lmaganda, namunaga ishlov berish suboptimal bo‘lganda, inhibitlovchi fiksatorlar mavjud bo‘lsa yoki EBV DNK miqdori tahlilning analitik sezgirligidan past bo‘lsa, yolg‘on-manfiy natijalar yuz berishi mumkin.
| O'lchov birligi | qualitative |
|---|---|
| Referens oraliq | — |
| Ko'rsatmalar | Suspected early infectious mononucleosis with compatible findings (eg, tonsillopharyngitis, hepatosplenomegaly, and cervical or perimandibular lymph node enlargement) and laboratory evidence of atypical lymphocytosis, Known HIV infection with concern for EBV-associated disease or reactivation, Post-transplant immunosuppression or other substantial immunosuppressive therapy with concern for EBV reactivation |
Natija og'ishlarining mumkin sabablari
Pasaygan daraja
- antiviral therapy
Namunangiz talablari
| Namunangiz | Namuna ko'rsatilmagan |
|---|---|
| Container | Testga qarab |
References
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