Eyakulyatda sperm DNK fragmentatsiyasi (SCD/Halosperm)
Kod:6033
Tahlil ma'lumotlari
Tadqiqot usuli
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Kutilayotgan natija topshirish vaqti
1 kun
Maxsus tayyorlik
- Avoid all alcohol for 6–7 days before specimen collection.
- Prevent any intoxication (tobacco, illicit drugs, workplace exposures, medications, or other toxins); if exposure occurs, schedule testing 5–10 days later.
- Do not take medications for 24 hours before testing unless approved by the treating clinician.
- The day before collection, avoid strenuous exercise and high-stress situations.
- Maintain sexual abstinence for at least 2 days and no more than 7 days prior to collection; use the same abstinence interval for repeat tests to limit variability.
- Avoid heat exposure (sauna/steam bath, occupational hyperthermia, or fever) for 7 days before testing.
- Do not undergo physiotherapy or radiographic studies during the 72 hours before collection.
- If a prostate massage was performed, wait 3–4 days before providing the specimen.
- After recovery from acute illnesses with fever, collect the sample 7–10 days later.
- After treatment of inflammatory disorders of the genitourinary tract, collect the sample after 2 weeks.
- Empty the bladder immediately before producing the semen sample.
- Do not use a condom or any lubricants, including saliva, for collection.
- Wash hands and external genitalia before masturbation.
- Collect the entire ejaculate.
Qanday foydalanish
Sperm DNK fragmentatsiyasi (SDF) sperm xromatin dispersiyasi (SCD) usuli orqali (Halosperm testi) ejakulyatdagi spermatozoidlarning yadroviy DNK yaxlitligini baholaydi. Tahlil DNKsi fragmentatsiyalangan spermatozoidlar ulushini aniqlaydi va erkakning fertilitet salohiyatini baholashni qo‘llab-quvvatlaydi. Bepusht juftlikning diagnostik tekshiruvlarida, IVF kabi yordamchi reproduktiv muolajalarni rejalash yoki optimallashtirishda hamda otalik genomik yaxlitligi e’tiborga olinadigan takroriy homiladorlik yo‘qotilishi bo‘yicha tekshiruvlarda qo‘llaniladi.
Cheklovlar
Taxminan 48.5 million juftlik dunyo bo‘ylab bepushtlikdan aziyat chekadi; holatlarning taxminan 20% dan 70% gachaida erkak omili aniqlanadi. Jahon sog‘liqni saqlash tashkiloti bepushtlikni kontratseptiyasiz muntazam jinsiy aloqadan so‘ng bir yil yoki undan ko‘proq muddatda homiladorlikka erishilmasligi sifatida ta’riflaydi. Shu kontekstdа sperm DNK fragmentatsiyasini o‘lchash kontseptsiya ehtimoli va homiladorlikni saqlanishiga ta’sir ko‘rsatishi mumkin bo‘lgan otalik genomik yaxlitligining ko‘rsatkichi sifatida ahamiyat kasb etdi. Sperm DNK yaxlitligi turli xil ta’sirlar va holatlar tufayli buzilishi mumkin, jumladan intoksikatsiyalar, kasbiy xavflar, noqulay atrof-muhit omillari, nosog‘lom turmush tarzi omillari, varikotsele, erkak reproduktiv yo‘llarining infeksiyalari va yallig‘lanish kasalliklari, sigaret chekish, narkotik moddalar iste’moli, isitmali kasalliklar hamda yoshning kattaligi. DNK shikastlanishi yuklamasi ortgani sayin genomik yaxlitlik pasayadi va homiladorlikka erishish ehtimoli kamayadi. DNKi shikastlangan spermatozoidlar oositni urug‘lantirishi mumkin bo‘lsa-da, yuqori darajadagi fragmentatsiya yordamchi reproduktiv urinishlarning muvaffaqiyatsizligi va tabiiy konsepsiyada takroriy tushishlar bilan bog‘liq. Spermadagi DNK shikastlanishi, shuningdek, onkogen jarayonlar xavfining ortishi va avlodga genetik nuqsonlarning uzatilishi bilan bog‘lanadi. Sperm xromatin dispersiyasi (SCD) testi ejakulyatni nazoratli ishlovdan so‘ng xromatin halosining hosil bo‘lishini vizuallashtirish orqali DNK fragmentatsiyasini baholaydi. Butun DNKga ega spermatozoidlar fermentativ ishlov va bo‘yashdan keyin dispersiyalangan DNK halqalarining yirik halolarini hosil qiladi, DNKi fragmentatsiyalangan spermatozoidlar esa juda kichik halolar yoki umuman halo hosil qilmaydi. Turli kohortalarda bepushtlik uchun baholangan erkaklarda, odatda, fertil nazoratlarga nisbatan SDF darajalari yuqoriroq bo‘ladi; bu testning erkak fertilitetini baholashdagi rolini qo‘llab-quvvatlaydi.
| Referens oraliq |
| ||||
|---|---|---|---|---|---|
| Ko'rsatmalar | Male partner evaluation in an infertile couple when pregnancy has not occurred after 12 months of regular unprotected intercourse (approximately 2–3 times per week)., Assessment in cases of recurrent pregnancy loss, defined as three or more consecutive spontaneous miscarriages before 22 weeks’ gestation., Workup after repeated failed in vitro fertilization attempts and during preparation for IVF., Evaluation when embryo abnormalities are documented during an IVF cycle. |
Namunangiz talablari
| Namunangiz | Sperma |
|---|---|
| Container | Steril idish |