Андрофлор

Androflor‑screen, a quantitative DNA real‑time PCR assay (Androflor PCR), profiles pathogens and conditionally pathogenic microorganisms in the male urogenital tract. The test establishes the infectious etiology, estimates microbial burden, and incorporates quantification of human genomic DNA to assess specimen adequacy. Results inform antimicrobial selection, enable monitoring of treatment effectiveness, and support longitudinal evaluation of the urogenital microbiota.

Infections and inflammatory conditions of the urogenital tract are a leading cause of impaired male reproductive function. Chronic inflammation can damage the seminiferous epithelium, disrupt the blood–testis barrier, and alter the rheology and biochemical composition of semen. Immune activation during these processes increases reactive oxygen species and cytokine production, which may sustain secondary inflammation and contribute to autoimmunity, including the formation of antisperm antibodies. Bacterial prostatitis is common across age groups and reflects inflammation of the prostate of infectious or physiologic origin, with reported frequencies ranging from 30% to 73%. Without timely intervention, the condition may progress: prostatic secretions continue to support microbial growth, and the gland’s limited blood flow reduces clearance, allowing accumulation of microbial by‑products and tissue congestion. Clinical manifestations can include dysuria with painful, difficult voiding and subsequent urinary retention lasting a day or longer. Traditional bacteriologic methods often require 7–14 days for results. By contrast, the Androflor real‑time PCR approach detects microbial DNA or RNA independent of culture or morphology, including uncultivable organisms; characterizes the microbiota structure to inform pathogenic roles; and provides quantitative data. The method delivers high analytical sensitivity and specificity with rapid amplification (approximately 1–1.5 hours), supports serial monitoring, and includes quantification of human genomic DNA. A single Androflor real‑time PCR panel consolidates multiple targeted assays and expands organism coverage to include obligate anaerobes.