Vitamin B12 (kobalamin)
Kod:12008|CPT:82607|LOINC:2132-9
| Kabi | Vitamin B12 |
|---|
Tahlil ma'lumotlari
Tadqiqot usuli
- Elektrokimyoluminessent immunoanaliz (ECLIA)
Kutilayotgan natija topshirish vaqti
1 kun
Maxsus tayyorlik
- Confirm whether the patient uses high-dose biotin; stop biotin supplements for at least 72 hours before collection.
- For infants younger than 1 year, withhold feeding for 30–40 minutes before the draw.
- Do not eat for 2–3 hours before specimen collection; water is allowed.
- Avoid physical exertion and emotional stress for 30 minutes before the blood draw.
- Do not smoke during the 30 minutes preceding collection.
Qanday foydalanish
Vitamin B12 testi (kobalaminni aniqlash) vitamin B12 yetishmovchiligini aniqlash, makrotsitozni (MCV >100 fL) va makrotsitar/megaloblastik anemiyani, jumladan pernisioz anemiyani tekshirish uchun qo‘llaniladi. U malabsorbsiyani va kobalamin yetishmovchiligi bilan mos keluvchi nevrologik namoyonlarni baholashni qo‘llab-quvvatlaydi hamda alkogoldan foydalanish buzilishi kontekstida va prenatal yoki homiladorlik rejalashtirish davrida tez-tez buyuriladi. Qon zardobida vitamin B12 ko‘tarilishi jigar hujayralari zararlanishi yoki miyeloid leykoz bilan birga kuzatilishi mumkin va klinik kontekstda talqin qilinishi lozim. Kobalamin uchun ko‘plab immunoanalizlar biotin–streptavidin kimyosidan foydalanganligi sababli, yuqori dozali biotin o‘lchangan konsentratsiyani sun’iy ravishda oshirishi mumkin. Namuna olishdan oldin belgilangan muddatga biotin qabulini to‘xtatish bu analitik xalaqit manbasini kamaytirishga yordam beradi.
Cheklovlar
Vitamin B12 yetishmovchiligi makrotsitoz va megaloblastik anemiya bilan bog‘liq bo‘lib, pernisioz anemiya ko‘rinishida namoyon bo‘lishi mumkin. Nevrologik shikoyatlar va belgilar kobalamin yetishmovchiligining xususiyatlari bo‘lishi mumkin, malabsorbsion sindromlar esa tekshiruv o‘tkazishga sabab bo‘ladigan keng tarqalgan klinik holatlardir. Tekshiruv alkogoldan foydalanish buzilishiga ega shaxslarda ovqatlanish holatini baholashda, shuningdek prenatal yoki homiladorlik rejalashtirish davrida ham qo‘llaniladi. Qon zardobidagi vitamin B12 yetishmovchilik bilan bog‘liq bo‘lmagan holatlarda, ayniqsa jigar hujayralari shikastlanishi yoki miyeloid leykozda oshgan bo‘lishi mumkin. Yuqori dozali biotindan kelib chiqadigan analitik xalaqit ayrim elektroxemiluminessent immunoanalizlarda asossiz ravishda yuqori natijalarni keltirib chiqarishi mumkin; namuna olishdan kamida 72 soat oldin biotin qabulini to‘xtatish ushbu ta’sirni kamaytiradi.
| O'lchov birligi | pg/mL | ||||
|---|---|---|---|---|---|
| Referens oraliq |
| ||||
| Ko'rsatmalar | Clinical or laboratory suspicion of vitamin B12 deficiency, including pernicious anemia, Evaluation of macrocytosis (MCV >100 fL), Workup of macrocytic or megaloblastic anemia, Assessment in suspected or known malabsorption, Neurologic symptoms potentially attributable to cobalamin deficiency, Nutritional assessment in individuals with alcohol use disorder, Screening or evaluation in prenatal or preconception settings |
Natija og'ishlarining mumkin sabablari
Oshgan daraja
- biotin (high dose)
Namunangiz talablari
| Namunangiz | Zardob |
|---|---|
| Container | Oltin/yo'lbars qopqoqli probirka (SST, gel ajratgich) |
| Hajm | 1 mL (min 0.7 mL) |
| Saqlash tayyorlik | Xona harorati, Sovutilgan, Muzlatilgan |
References
A. Sh. Byshevsky, O. A. Tersenov. Biochemistry for the Physician. Yekaterinburg, 1994.
A. Sh. Zaichik, L. P. Churilov. Fundamentals of Pathochemistry. St. Petersburg, 2001.
Shelke N, Keith L. Folic Acid Supplementation for Women of Childbearing Age versus Supplementation for the General Population: A Review of the Known Advantages and Risks. Int J Family Med. 2011.