Oddiy gerpes virusi (HSV) 1/2 tiplari uchun antitanachalar, IgM, immunoblot
Kod:17008
| Kabi | HSV-1 ga qarshi IgM antitanachalar HSV-2 ga qarshi IgM antitanachalar |
|---|
Tahlil ma'lumotlari
Tadqiqot usuli
- Western blot
Kutilayotgan natija topshirish vaqti
1 kun
Maxsus tayyorlik
- Do not eat for 2–3 hours before the blood draw; water is allowed.
- Avoid smoking for at least 30 minutes prior to specimen collection.
Qanday foydalanish
Oddiy gerpes virusi (HSV) 1/2 tiplari uchun antitanachalar, IgM, immunoblot HSV 1/2 bilan bog‘liq kasallikni baholash va HSV IgM yoki IgG bo‘yicha immunoferment tahlilning noaniq yoki sust-musbat topilmalarini tasdiqlash uchun qo‘llanadi. Test erta infeksiya gumon qilingan klinik holatlarda diagnostik baholashga yordam beradi, jumladan HSV ensefaliti, intrauterin fetal infeksiya va disseminatsiyalangan gerpesvirus infeksiyasi. Ushbu baholash uchun HSV 1/2 va oddiy gerpes virusi 1 va 2 tiplari kabi sinonimlar keng qo‘llanadi.
Cheklovlar
HSV-1 va HSV-2 qobiq bilan qoplangan DNK viruslari bo‘lib, sezgi gangliyalarida umrboqiy latentlikni o‘rnatadi va vaqti-vaqti bilan reaktivatsiyalanadi. Yuqtirish nafas yo‘li tomchilari, bevosita kontakt, jinsiy aloqa va platsenta orqali sodir bo‘ladi. Klinik ko‘rinishlar gingivostomatit, genital gerpes, keratokonyunktivit (ko‘proq HSV-1 bilan bog‘liq), ensefalit, intrauterin infeksiya, homila yo‘qolishi va disseminatsiyalangan neonatal kasallikni (odatda HSV-2 bilan bog‘liq) o‘z ichiga oladi. Homiladorlikning ikkinchi yoki uchinchi trimestrida onadagi birlamchi infeksiya homila infeksiyalanish ehtimolini oshiradi. HSV teratogen hisoblanmaydi va klinik oqibatlar boshqa TORCH patogenlaridan farq qiladi. Immunoblot metodologiyasi alohida HSV antigenlariga qarshi antitanachalarni kuchli analitik ko‘rsatkichlar bilan aniqlaydi, skrining immunoferment analiz natijalarini tasdiqlaydi, antigen reaktivlik naqshlariga asoslangan tipga xos talqinni (masalan, gG-ga asoslangan javoblar) qo‘llab-quvvatlaydi va infeksiya bosqichini aniqlash hamda subklinik HSV-1 va HSV-2 ni tanishda yordam beradi.
| O'lchov birligi | qualitative | ||||
|---|---|---|---|---|---|
| Referens oraliq |
| ||||
| Ko'rsatmalar | Evaluation of suspected HSV meningoencephalitis., First episode of genital herpes occurring in the second or third trimester of pregnancy., Recurrent genital herpes during the second or third trimester of pregnancy., Workup for possible intrauterine HSV infection with features such as fetal growth restriction, hepatosplenomegaly, or hydrocephalus., Concern for disseminated HSV in immunocompromised individuals, pregnant patients, or neonates., Confirmation of equivocal or weakly positive HSV IgM or IgG enzyme immunoassay results., Inclusion in the differential diagnosis of urogenital infections., Preconception assessment when a partner may have been exposed to HSV., Assessment for intrauterine infection in the setting of placental insufficiency., Testing in the context of HIV infection or other immunodeficiency states. |
Namunangiz talablari
| Namunangiz | Zardob |
|---|---|
| Container | Oltin/yo'lbars qopqoqli probirka (SST, gel ajratgich) |
| Saqlash tayyorlik | Sovutilgan, Muzlatilgan |
References
Biasi RL, Kleinschmidt-DeMasters BK, Weinberg A, Tyler KL. Use of PCR for the diagnosis of herpesvirus infections of the central nervous system. J Clin Virol. 2002 Jul;25 Suppl 1:S5-11.
Espy MJ, Uhl JR, Mitchell PS, Thorvilson JN, Svien KA, Wold AD, Smith TF. Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR. J Clin Microbiol. 2000 Feb;38(2):795-9.
Avgil M, Ornoy A. Herpes simplex virus and Epstein-Barr virus infections in pregnancy: consequences of neonatal or intrauterine infection. Reprod Toxicol. 2006 May;21(4):436-45.