Qaytarish

Vitamin D, 25-gidroksi

Kod:12009|CPT:82306|LOINC:62292-8

Sinonimlar
Витамин Д25-гидроксивитамин D25-гидроксикальциферол25(OH)D25-OH-D25-hydroxycalciferol25-hydroxyvitamin Dcalcidiol (25-hydroxy-vitamin D)calcifidiol (25-hydroxy-vitamin D)cholecalciferol metabolitevitamin Dvitamin D, 25-hydroxyvitamin D3 metabolite
KabiVitamin D, 25-gidroksi

Tahlil ma'lumotlari

Tadqiqot usuli

  • Immunokimyoluminometrik analiz (ICMA)
  • Suyuq xromatografiya va tandem mass-spektrometriya (LC-MS/MS)

Kutilayotgan natija topshirish vaqti

1 kun

Maxsus tayyorlik

  • Infants (<1 year): do not feed for 30–40 minutes before the blood draw.
  • Children 1–5 years: fast for 2–3 hours before specimen collection.
  • Older children and adults: avoid food for 2–3 hours before collection; water is allowed.
  • Do not smoke during the 30 minutes preceding collection.
  • This assay is different from calcitriol (1,25‑dihydroxyvitamin D); order that test separately if required.

Qanday foydalanish

Vitamin D, 25-gidroksi tahlili (25[OH]D; kalsidiol; 25-gidroksikalsiferol) vitamin D yetishmovchiligini tashxislash va ortiqcha qo‘shimcha qabulini aniqlash, shuningdek kaltsiy–fosfat almashinuvi buzilishlari va skelet kasalliklarini baholash uchun qo‘llaniladi. Shuningdek, vitamin D terapiyasiga javobni monitoring qilish va almashtirish davolashida doza tuzatmalarini yo‘naltirish uchun ishlatiladi. Klinik amaliyotda 25(OH)D o‘lchovi gipokalsemiya bilan birga paratireoid gormoni konsentratsiyalarining g‘ayritabiiyligi bo‘lganda diagnostik izlanish tarkibiga kiritiladi, osteoporozga qarshi davolashni boshlashdan oldin boshlang‘ich ko‘rsatkich sifatida va malabsorbsiya sindromlari bo‘lgan bemorlarda qo‘llanadi.

Cheklovlar

Vitamin D yog‘da eruvchan prohormon bo‘lib, zardobdagi kaltsiy, fosfor va magniy gomeostazini tartibga soladi. Xolekalsiferol (D3) yoki ergokalsiferol (D2) ning jigarda aylanishi natijasida 25-gidroksivitamin D [25(OH)D] hosil bo‘ladi; bu aylanmadagi asosiy shakl bo‘lib, faol gormon bilan solishtirganda yuqoriroq konsentratsiyada aylanadi va yarim parchalanish davri uzoqroq, shu bois vitamin D holatining eng ishonchli ko‘rsatkichi hisoblanadi. Biologik faol metabolit — 1,25-gidroksivitamin D — buyrakda hosil bo‘ladi. Endogen xolekalsiferol ultrabinafsha B nurlanishi ta’sirida terida sintezlanadi, ekzogen manbalarga esa yog‘li baliqlar va baliq jigar yog‘lari kabi ratsion mahsulotlari kiradi. Yetishmovchilik skelet mineralizatsiyasini buzadi, bolalarda raxit, kattalarda osteomalatsiyani keltirib chiqaradi va osteoporoz xavfiga hissa qo‘shadi. Malabsorbsiya, jigar kasalligi, nefrotik sindrom, quyosh nurlariga cheklangan ta’sir yoki keksalik bo‘lgan shaxslar yuqori xavf ostida. Ortiqcha qo‘shimcha qabul qilish giperkalsemiya va bog‘liq a’zolar shikastlanishi bilan kechadigan toksiklikka olib kelishi mumkin. O‘lchangan 25(OH)D konsentratsiyalari quyosh nurlariga ta’sirlanishga qarab o‘zgaradi. Ba’zi testlar boshqa metabolitlarning kichik miqdorlarini (masalan, 24,25-gidroksivitamin D) aniqlaydi, biroq ularning 25(OH)D ga nisbatan past fiziologik darajalari vitamin D holatini baholashga mazmunli ta’sir ko‘rsatmaydi.

O'lchov birliging/mL
Referens oraliq
MinMaks
30100
Ko'rsatmalarSuspected vitamin D deficiency, including rickets in children and osteomalacia in adults, Broad assessment of calcium–phosphate homeostasis, Hypocalcemia and/or an abnormal parathyroid hormone level, Baseline testing prior to starting osteoporosis therapy, Malabsorption conditions (e.g., cystic fibrosis, Crohn disease, celiac disease), Tracking response during vitamin D supplementation

Natija og'ishlarining mumkin sabablari

Pasaygan daraja

  • oral anticoagulants
  • phenobarbital
  • phenytoin
  • rifampin

Namunangiz talablari

NamunangizZardob
ContainerOltin/yo'lbars qopqoqli probirka (SST, gel ajratgich)
Hajm0.5 mL (min 0.3 mL)
Saqlash tayyorlikXona harorati, Sovutilgan, Muzlatilgan

References

Endres DB, Rude RK. Mineral and Bone Metabolism. In: Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical Chemistry. 3rd ed. Philadelphia, PA: WB Saunders; 1999:1395–1457.

Holick MF, Binkley NC, Bischoff-Ferrare HA, et al. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2011;96(7):1911–1930.

Souberbielle JC, Body JJ, Lappe JM, et al. Vitamin D and Musculoskeletal Health, Cardiovascular Disease, Autoimmunity and Cancer: Recommendations for Clinical Practice. Autoimmun Rev. 2010;9(11):709–715.

Fischbach FT, Dunning MB. A Manual of Laboratory and Diagnostic Tests. 8th ed. Lippincott Williams & Wilkins; 2008.

Raine JE, et al. Practical Endocrinology and Diabetes in Children. 2nd ed. Blackwell Publishing; 2006.

Nazarenko GI, Kishkun A. Clinical Evaluation of Laboratory Test Results. Moscow: Meditsina; 2000.