To'qima transglutaminazaga (tTG) qarshi antitanachalar, IgA
Kod:16024|CPT:86364|LOINC:31017-7
| Kabi | To'qima transglutaminazaga (tTG) qarshi IgA |
|---|
Tahlil ma'lumotlari
Tadqiqot usuli
- Ferment immunoanalizi (EIA)
- Kimyoluminessent immunoanaliz (CLIA)
Kutilayotgan natija topshirish vaqti
1 kun
Maxsus tayyorlik
- Do not smoke for at least 30 minutes before the blood draw.
Qanday foydalanish
To'qima transglutaminazaga (tTG) qarshi IgA antitanachalar testi (anti‑tTG IgA yoki transglutaminazaga qarshi antitanachalar, IgA nomlari bilan ham yuritiladi) seliak kasalligini va dermatit herpetiformisni tashxislashga yordam beradi hamda kimlarda endoskopik ingichka ichak tekshiruvi o'tkazilishi kerakligini aniqlashga ko'maklashadi. Shuningdek, u yuqori xavf guruhlarida, masalan seliak kasalligi oilaviy anamnezi bo'lganlar, 1-tur qandli diabet yoki Daun sindromi bo'lgan shaxslarda, maqsadli skrining uchun qo'llanadi. Vaqt bo'yicha ketma-ket o'lchashlar antitanachalar kontsentratsiyasining vaqt o'tishi bilan kamayishini kuzatish orqali glyutensiz parhezga javob monitoringini qo'llab-quvvatlaydi.
Cheklovlar
To'qima transglutaminaza glutamin va lizin bilan boy oqsillar o'rtasida kross-bog'lanish reaksiyalarini katalizlaydi va bir nechta to'qimalarda, jumladan ichak submukozasida, mavjud. Qabul qilingan glyuten tTG tomonidan deamidatsiyalanadigan gliadin peptidlarini taqdim etadi, bu ularning immunogenligini oshiradi. Genetik moyilligi bo'lgan shaxslarda ushbu jarayon kichik ichak shilliq qavatiga zarar yetkazuvchi autoimmun javobni qo'zg'atadi, natijada villoz atrofiyasi va malabsorbsiyaga olib keladi. tTG IgA bilan serologik tekshiruv seliak kasalligi uchun yuqori diagnostik aniqlikni ko'rsatadi; sezuvchanlik taxminan 81%–100% va xoslik taxminan 97%–99%, hamda endoskopik ingichka ichak biopsiyasiga nomzodlarni aniqlashga yordam beradi. Seliak kasalligi bo'lgan bemorlar orasida selektiv IgA yetishmovchiligi ko'proq uchraydi, shuning uchun IgA yetishmovchiligi mavjud bo'lganda umumiy IgA ni o'lchash va IgG-asosli testlardan (masalan, tTG IgG) foydalanish tavsiya etiladi. Qat'iy glyutendan voz kechilganda, tTG IgA kontsentratsiyalari odatda 6–12 oy davomida kamayadi, bu glyutensiz parhezga rioya qilinishini ob'yektiv baholashga imkon beradi. Seliak kasalligi minimal simptomli bo'lishi mumkin; shu sababli xavf guruhlarida, jumladan birinchi darajali qarindoshlar va 1-tur qandli diabet, autoimmun tireoidit yoki Daun sindromi bo'lgan shaxslar, davriy serologik skrining ko'rib chiqiladi.
| O'lchov birligi | IU/mL | ||||
|---|---|---|---|---|---|
| Referens oraliq |
| ||||
| Ko'rsatmalar | Assessment of suspected celiac disease in patients with chronic diarrhea, Recurrent episodes of vomiting, Poor linear growth or failure to thrive in children, Chronic or recurrent abdominal pain, Unexplained iron deficiency anemia, Epilepsy, Atopic dermatitis, Angular cheilitis, Recurrent aphthous ulcers of the mouth, Dermatitis herpetiformis (Duhring disease), Visual disturbances, Peripheral neuropathy, Osteopenia, Bleeding tendency (bleeding diathesis), Amenorrhea, Infertility, Erectile dysfunction, Evaluation of individuals with a family history of celiac disease, Monitoring treatment response in celiac disease, Screening in individuals with type 1 diabetes mellitus, Screening in patients with Hashimoto thyroiditis, Screening in alopecia totalis, Screening in systemic connective tissue disorders, Screening in individuals with Down syndrome |
Natija og'ishlarining mumkin sabablari
Pasaygan daraja
- age under 3 years
- gluten-free diet before testing
- selective iga deficiency
Namunangiz talablari
| Namunangiz | Zardob |
|---|---|
| Container | Oltin/yo'lbars qopqoqli probirka (SST, gel ajratgich) |
| Hajm | 1 mL (min 0.5 mL) |
| Saqlash tayyorlik | Xona harorati, Sovutilgan, Muzlatilgan |
References
Dahele AV, Aldhous MC, Humphreys K, Ghosh S. Serum IgA tissue transglutaminase antibodies in coeliac disease and other gastrointestinal diseases. QJM. 2001 Apr;94(4):195-205.
Armstrong D, Don-Wauchope AC, Verdu EF. Testing for gluten-related disorders in clinical practice: the role of serology in managing the spectrum of gluten sensitivity. Can J Gastroenterol. 2011 Apr;25(4):193-7.
Fauci AS, Kasper DL, Longo DL, Braunwald E, Hauser SL, Jameson JL, Loscalzo J, editors. Harrison's Principles of Internal Medicine. 17th ed. New York: McGraw-Hill; 2008.
Dahlbom I, Olsson M, Forooz NK, Sjöholm AG, Truedsson L, Hansson T. Immunoglobulin G (IgG) anti-tissue transglutaminase antibodies used as markers for IgA-deficient celiac disease patients. Clin Diagn Lab Immunol. 2005 Feb;12(2):254-8.