Фемофлор 16, мазок

Femoflor 16 Real Time PCR is a real-time PCR panel that profiles the urogenital microbiota in women, reporting both qualitative and quantitative features, including total bacterial mass, Lactobacillus status, and groups of opportunistic organisms (e.g., mycoplasmas, ureaplasmas, Candida). The assay supports evaluation of women with clinical or laboratory evidence of urogenital inflammation and characterization of dysbiosis using a molecular approach. As a vaginal microbiota PCR (vaginal biocenosis PCR), it is used in preconception assessment, including preparation for assisted reproductive technologies, and in preoperative gynecologic evaluation. The test also serves for longitudinal monitoring to document treatment response and restoration of a normal vaginal microbiome (normocenosis).

Human microbial communities are concentrated in the skin, gastrointestinal tract, and urogenital tract, with the normal female urogenital flora typically dominated by Lactobacillus species that help prevent pathogen colonization. Disturbance of this ecosystem can alter both the composition and abundance of key taxa, resulting in inflammation. In many cases the process is polymicrobial and reflects shifts in the normal flora rather than a single pathogen, often presenting with nonspecific or mild symptoms and a tendency toward chronicity, which increases the risk of secondary infections and reproductive complications. Because the organisms most frequently implicated in dysbiosis-related urogenital inflammation are anaerobes, culture-based detection is technically challenging and not widely available. Real-time PCR addresses these limitations by detecting microbial DNA, amplifying target sequences, and relating the cycle thresholds to the starting DNA amount, thereby enabling quantitative estimation of organism burden. Femoflor-16 applies this real-time PCR (RT-PCR) methodology to characterize vaginal biocenosis in women. The panel incorporates an internal human DNA control for specimen adequacy, measures total bacterial mass, quantifies Lactobacillus, and detects DNA from 23 groups of conditionally pathogenic microorganisms, yielding 25 reported parameters. These data provide an integrated view of qualitative and quantitative shifts in the microbiota that can inform antimicrobial and adjunctive therapy. A lower-than-threshold total bacterial mass (overall bacterial load) indicates insufficient colonization of the site, which may follow recent antibiotic exposure or reflect hormonal imbalance. For optimal diagnostic yield, testing is preferably performed after excluding infections caused by recognized urogenital pathogens such as Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis. To shorten the diagnostic timeline, specimens for STI testing and Femoflor-16 may be collected in parallel.