Бак. посев отделяемого из коньюктивы глаза (правого) на микрофлору с определением чувствительности к антибиотикам

Culture for aerobic and facultative anaerobic flora with antibiotic susceptibility testing and MIC determination recovers clinically significant aerobic and facultative anaerobic bacteria from a variety of specimens, then characterizes antimicrobial response. Use of this aerobic culture (culture and sensitivity, aerobic) aids differentiation of aerobic versus anaerobic infections and supports evaluation of chronic, low-grade, or persistent infections, including organisms that are difficult or not amenable to routine cultivation. Results guide targeted therapy by identifying an effective antibiotic and concentration.

Anaerobic microorganisms do not require oxygen for growth; for many, oxygen exposure is lethal. They are part of normal human microbiota in the gastrointestinal, respiratory, and genitourinary tracts. Infection may arise endogenously when immunity is reduced or tissue integrity is disrupted, and less commonly from exogenous inoculation (eg, deep puncture wounds, septic abortion, thoracic or abdominal trauma, implantation of pins or prostheses). In soft tissues, anaerobes may cause cellulitis, abscesses, and myositis. Findings that raise concern for anaerobic infection include tense edema, gas in tissues producing crepitus, foul odor, and putrid drainage. Management of anaerobic soft‑tissue infection is primarily surgical, with source control and wound opening to permit oxygen exposure, which is detrimental to obligate anaerobes. In contrast, aerobic bacteria require oxygen, using it for energy production; as prokaryotes they lack a true nucleus and typically propagate by division or budding, generating toxic oxidative by‑products. Successful cultivation of aerobic organisms depends on appropriate media, control of oxygen tension, and maintenance of optimal temperatures. Each species has a characteristic range of oxygen concentrations that supports growth. Facultative anaerobes perform anaerobic metabolic pathways yet remain capable of survival and proliferation in the presence of oxygen, distinguishing them from obligate anaerobes. To separate aerobic from anaerobic etiologies and inform management, clinical specimens are cultured to isolate the causative organisms, followed by species identification. After growth is obtained, antibiotic susceptibility testing is performed, including determination of the minimum inhibitory concentration. Because antimicrobial resistance is increasingly prevalent, choosing therapy solely by expected spectrum may be ineffective; phenotypic susceptibility testing helps identify the specific agent most likely to succeed in the individual case.